Trypsin Properties

Human trypsin has an optimal operating temperature of about 37 °C. In contrast, the Atlantic cod has several types of trypsins in adjustment for the poikilotherm angle to survive at altered physique temperatures. Cod trypsins cover trypsin I with an action ambit of 4 to 65 °C (40 to 150 °F) and acute action at 55 °C (130 °F), as able-bodied as trypsin Y with a ambit of 2 to 30 °C (36 to 86 °F) and a acute action at 21 °C (70 °F).

As a protein trypsin has assorted atomic weights depending on the source. For example, a atomic weight of 23.3 kDa is appear for trypsin from bovine and beefy sources.

The action of trypsin is not afflicted by the agitator inhibitor tosyl phenylalanyl chloromethyl ketone, TPCK, which deactivates chymotrypsin. This is important because, in some applications, like accumulation spectrometry, the specificity of break is important.

Trypsin should be stored at actual algid temperatures (between −20 °C and −80 °C) to anticipate autolysis, which may aswell be impeded by accumulator of trypsin at pH 3 or by application trypsin adapted by reductive methylation. When the pH is adapted aback to pH 8, action returns.